一、產(chǎn)品介紹 Product introduction

Bionoon-64P非接觸式超聲波DNA打斷儀，采用等溫、非接觸的方式，可隔著離心管一次性對(duì)64個(gè)樣品進(jìn)行打斷、勻漿和混合?？捎糜跓o(wú)菌、超微量破碎以及染色體打斷，專(zhuān)為二代測(cè)序DNA樣本及染色質(zhì)免疫共沉淀實(shí)驗(yàn)樣品前處理定制，最少可處理5UL微量樣品，適用于珍貴樣本。

Bionoon-64P Non-contact ultrasonic DNA interrupter uses an isothermal, non-contact method to break, homogenize and mix 64 samples at a time through a centrifuge tube. It can be used for asepsis, ultramicro breakage and chromosome breakage, specially customized for the pre-treatment of second-generation sequencing DNA samples and chromatin immunoprecipitation experiment samples, at least 5UL trace samples, suitable for precious samples.

二、產(chǎn)品特點(diǎn) Product feature

1.高通量：一次性可最多同時(shí)處理64個(gè)樣品，實(shí)驗(yàn)效率高。

1. High throughput: up to 64 samples can be processed simultaneously at one time, and the experimental efficiency is high.

2.避免交叉污染：無(wú)需頻繁操作探頭，各種樣品在單獨(dú)的全封閉試管內(nèi)，避免交叉污染。同時(shí)，在密閉的環(huán)境下，可有效避免超聲過(guò)程中產(chǎn)生的氣霧或者泡沫擴(kuò)散到環(huán)境中而造成的潛在生物危險(xiǎn)。

2. Avoid cross contamination: there is no need to operate the probe frequently, and various samples are in a separate fully enclosed test tube to avoid cross contamination. At the same time, in a closed environment, it can effectively avoid the potential biological hazards caused by the diffusion of aerosol or foam generated during the ultrasonic process into the environment.

3.重復(fù)性好、準(zhǔn)確性高：超聲參數(shù)設(shè)置靈活，實(shí)驗(yàn)步驟標(biāo)準(zhǔn)化，實(shí)驗(yàn)重復(fù)性好，結(jié)果可靠性高。

3. Good repeatability and high accuracy: ultrasonic parameter setting is flexible, experimental steps are standardized, experimental repeatability is good, and the results are highly reliable.

4.樣品保護(hù)性好：采用4℃低溫水浴超聲波，能量分布均勻，超聲作用完全，在低溫環(huán)境中，避免樣品的變性。

4. Good sample protection: The use of 4℃ low temperature water bath ultrasound, uniform energy distribution, ultrasonic effect is complete, in low temperature environment, to avoid sample denaturation.

5.配置豐富：可配置0.1ml-15ml多種適配器，滿(mǎn)足多種樣品實(shí)驗(yàn)需求；

5. Rich configuration: 0.1ml-15ml variety of adapters can be configured to meet the needs of a variety of samples;

6.實(shí)驗(yàn)成本低：無(wú)需特殊專(zhuān)用耗材，實(shí)驗(yàn)成本相對(duì)低。

6. Low experimental cost: no need for special consumables, the experimental cost is relatively low.

三、工作原理  Working principle

通過(guò)壓電轉(zhuǎn)換器，將電信號(hào)轉(zhuǎn)變?yōu)楦哳l聲波信號(hào)，產(chǎn)生數(shù)百萬(wàn)的分子“空穴”，利用“空穴”在幾秒內(nèi)變大、破裂產(chǎn)生的瞬時(shí)流體剪切力，通過(guò)等溫、非接觸的方式對(duì)樣本進(jìn)行打斷、勻漿和混合。

Through piezoelectric converters, electrical signals are converted into high-frequency acoustic signals, creating millions of molecular "holes" that utilize "voids.The instantaneous fluid shear force generated by the hole's enlarging and breaking in a few seconds interrupts, homogenizes, and mixes the sample in an isothermal, non-contact manner.

四、應(yīng)用領(lǐng)域   Application fields

■DNA片段化   DNA fragmentation

■RNA 片段化   RNA fragmentation

■ChIP assay（染色質(zhì)免疫共沉淀）樣本前處理

ChIP assay (Chromatin immunoprecipitation) sample pretreatment

■細(xì)菌、細(xì)胞的破碎及提取膜蛋白

Fragmentation of bacteria and cells and extraction of membrane proteins

■霉菌孢子等的破碎、均質(zhì)、乳化反應(yīng)等

Crushing, homogenization, emulsification of mold spores, etc

■貴重試劑的超聲處理   Ultrasonic treatment of valuable reagents

■高通量測(cè)序儀樣本前處理  High-throughput sequencer sample preprocessing

五、技術(shù)參數(shù) Technical parameters

1. 采用超聲波聚焦原理對(duì)樣品進(jìn)行破碎； 

 The sample is broken by ultrasonic focusing principle;

2. 破碎細(xì)胞細(xì)菌全過(guò)程樣品均處于完全封閉狀態(tài)，避免樣品交叉污染；

The whole process of broken cell bacteria samples are in a completely closed state to avoid cross-contamination of samples;

3. 超聲波DNA打斷儀適用于超微量EP管，最低可處理5ul的樣品，適用于珍貴樣品；

 Ultrasonic DNA interrupter is suitable for ultra-trace EP tube, minimum can handle 5ul samples, suitable for precious samples;

4. 可用于測(cè)序DAN樣本和CHIP染色體免疫共沉淀實(shí)驗(yàn)樣本前處理；

 It can be used for pre-processing of sequencing DAN samples and CHIP chromosome immunoprecipitation samples;

5. 核酸樣本處理范圍100bp-1kb；Nucleic acid sample processing range 100bp-1kb;

6. 樣品不接觸超聲波探頭，全程在封閉式管內(nèi)破碎；

The sample does not touch the ultrasonic probe and is broken in the closed tube throughout;

7. 控制方式：中英文同步觸摸屏操作 相位/頻率/波形實(shí)時(shí)顯示；

Control mode: real-time display of synchronous touch screen operation phase/frequency/waveform;

8. 控制屏尺寸：≥7寸觸摸屏；Control screen size: ≥7 inches touch screen;

9. 頻率控制：自動(dòng)追蹤；Frequency control: automatic tracking;

10. 溫度系統(tǒng)：溫度探頭實(shí)時(shí)探測(cè)冰浴，過(guò)溫自動(dòng)保護(hù)提醒；

Temperature system: temperature probe real-time detection of ice bath, over temperature automatic protection reminder;

11. 處理量：最多64個(gè)樣品（200ul）； Processing capacity: up to 64 samples (200ul);

12. 雙語(yǔ)支持：中英文桌面同時(shí)顯示； Bilingual support: both English and Chinese desktop display;

13. 振幅可調(diào)：1-100%；Adjustable amplitude: 1-100%;

14. 數(shù)據(jù)保存：最多可保存20個(gè)數(shù)據(jù)； Data preservation: up to 20 data can be saved;

15. 振幅調(diào)節(jié)：0-100%； Amplitude adjustment: 0-100%;

16. 相位/頻率: 同時(shí)顯示；Phase/frequency: display simultaneously;

17．功率可調(diào)：1-100%（總功率1000W）； Power adjustable: 1-100% (total power 1000W);

18. 制冷系統(tǒng)：外置配套；Refrigeration system: external supporting;

19. 制冷溫度：-5℃至100℃；Cooling temperature: -5℃ to 100℃;

20. 時(shí)間控制：工作時(shí)間0.1s-999min任意可調(diào)；間歇時(shí)間0.1s-999m任意可調(diào)；

Time control: working time 0.1S-999min can be adjusted; Intermittent time 0.1S-999M adjustable;

21. 工作槽尺寸 Working slot size：240x150x100mm；

22. 整機(jī)外殼材質(zhì)：采用開(kāi)模ABS塑殼一體成型；

The shell material of the whole machine: the ABS plastic shell with open mold is formed;

23. 整機(jī)外形尺寸 Overall dimensions (L*W*H)：578*360*370mm.

六、配置清單 Configuration list

1、主機(jī)1套（含冷卻循環(huán)系統(tǒng)）；

The main machine 1 set (including cooling circulation system);

2. 200ul適配器/單次可最多處理樣品64個(gè)；

200ul adapter/can handle up to 64samples at a time;

3. 500ul適配器/單次可最多處理樣品32個(gè); 

500ul adapter/can handle up to 32samples at a time;

4. 1.5/2ml適配器/單次可最多處理樣品20個(gè); 

1.5/2ml adapter/can handle up to 20 samples at a time;

5. 5ml適配器/單次可最多處理樣品10個(gè)。

5ml adapter/can handle up to 10 samples at a time.